(Flow cytometry , FCM ) yog lub ntsuas hluav taws xob ntawm tes uas ntsuas qhov fluorescence siv ntawm cov cim ntawm tes.Nws yog lub tshuab thev naus laus zis uas tsim los ntawm kev tshuaj xyuas thiab kev txheeb xyuas ntawm ib lub hlwb.Nws tuaj yeem ntsuas thiab faib qhov loj me, cov qauv sab hauv, DNA, RNA, cov proteins, antigens thiab lwm yam khoom ntawm lub cev lossis tshuaj lom neeg ntawm cov hlwb, thiab tuaj yeem ua raws li kev sau ntawm cov kev faib tawm no.
Flow cytometer feem ntau muaj tsib ntu hauv qab no:
1 Flow chamber thiab fluidics system
2 Laser lub teeb qhov chaw thiab beam shaping system
3 Optical system
4 Electronics, cia, tso saib thiab tsom xam qhov system
5 Cell sorting system
Ntawm lawv, laser excitation nyob rau hauv lub laser lub teeb qhov chaw thiab beam forming system yog lub ntsiab ntsuas ntawm fluorescence signals nyob rau hauv txaus cytometry.Qhov kev siv ntawm lub teeb excitation thiab lub sij hawm raug muaj feem xyuam rau qhov siv ntawm lub teeb liab fluorescence.Laser yog ib lub teeb ci sib txuas uas tuaj yeem muab ib lub wavelength, siab siv, thiab siab ruaj khov illumination.Nws yog qhov zoo tshaj plaws excitation teeb qhov chaw kom ua tau raws li cov cai no.
Muaj ob lub cylindrical lo ntsiab muag ntawm lub laser qhov chaw thiab lub ntws chamber.Cov lo ntsiab muag no tsom ib lub teeb laser nrog lub voj voog hla ntu tawm los ntawm lub laser qhov chaw mus rau hauv ib qho elliptical beam nrog ib qho me me hla ntu (22 μm × 66 μm).Lub zog laser nyob rau hauv lub elliptical beam no yog muab faib raws li ib txwm tis, kom ntseeg tau zoo ib yam illumination siv rau cov hlwb dhau los ntawm lub laser nrhiav kom tau qhov chaw.Ntawm qhov tod tes, lub tshuab kho qhov muag muaj ntau yam ntawm lo ntsiab muag, pinholes, thiab cov ntxaij lim dej, uas tuaj yeem muab faib ua ob pawg: sab saud thiab qis ntawm cov dej ntws.
Lub optical system nyob rau hauv pem hauv ntej ntawm lub flow chamber muaj ib lub lens thiab pinhole.Lub luag haujlwm tseem ceeb ntawm lub lens thiab pinhole (feem ntau yog ob lub lo ntsiab muag thiab lub pinhole) yog tsom rau lub teeb ci laser nrog lub voj voog hla ntu tawm los ntawm laser qhov chaw mus rau hauv lub teeb elliptical nrog ib qho me me cross-section.Qhov no faib lub zog laser raws li kev faib tawm ib txwm, ua kom lub teeb pom kev zoo sib xws rau cov hlwb thoob plaws hauv cheeb tsam laser pom thiab txo qis kev cuam tshuam los ntawm lub teeb tsis pom kev.
Muaj peb hom kev lim dej loj:
1: Ntev dhau lim (LPF) - tsuas yog tso cai rau lub teeb nrog wavelengths siab tshaj tus nqi tshwj xeeb kom dhau mus.
2: Short-pass lim (SPF) - tsuas yog tso cai rau lub teeb nrog wavelengths hauv qab tus nqi tshwj xeeb kom dhau mus.
3: Bandpass lim (BPF) - tsuas yog tso cai rau lub teeb nyob rau hauv ib qho kev sib tw wavelength kom dhau mus.
Kev sib xyaw ua ke ntawm cov lim dej sib txawv tuaj yeem coj cov teeb liab fluorescence ntawm qhov sib txawv wavelengths rau ib tus neeg photomultiplier hlab (PMTs).Piv txwv li, cov ntxaij lim dej kom pom cov fluorescence ntsuab (FITC) nyob rau hauv pem hauv ntej ntawm PMT yog LPF550 thiab BPF525.Cov ntxaij lim dej siv los kuaj cov txiv kab ntxwv-liab fluorescence (PE) nyob rau hauv pem hauv ntej ntawm PMT yog LPF600 thiab BPF575.Cov lim dej kom pom cov fluorescence liab (CY5) nyob rau hauv pem hauv ntej ntawm PMT yog LPF650 thiab BPF675.
Flow cytometry feem ntau yog siv rau kev txheeb xyuas ntawm tes.Nrog rau kev nce qib ntawm kev siv tshuab computer, kev txhim kho ntawm kev tiv thaiv kab mob thiab kev tsim kho ntawm monoclonal antibody technology, nws cov kev siv hauv biology, tshuaj, khw muag tshuaj thiab lwm yam kev lag luam tau nce zuj zus.Cov ntawv thov no suav nrog kev txheeb xyuas cov cell dynamics, cell apoptosis, cell typing, qog diagnosis, tshuaj xyuas kev siv tshuaj, thiab lwm yam.
Post lub sij hawm: Sep-21-2023
